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. 2018 Apr 29;2018:4126379. doi: 10.1155/2018/4126379

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Copyright © 2018 Ilaria Roato et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Osteoblast differentiation of ASCs and SVF on SmartBone. At 60 days, in ctrl condition, collagen I (COLL I) was weakly positive on SmartBone cultured with ASCs (a), whereas in culture with SVF, COLL I was expressed (c). Both in cultures with ASCs and SVF with osteogenic medium (OM), COLL I markedly stained the new tissue on SmartBone (b, d). Osteocalcin (OCN) was weakly positive in ctrl (e, g), and it was highly expressed with OM, mainly on the periphery of the newly formed tissue (f, h). TGFb stained osteoblasts at the margin of the newly formed tissue both with and without OM, with ASCs and SVF (i–l). Magnification: 20x.