Figure 8.

Secretion of ET1 and VEGF by ASCs and SVF plated on SB by ELISA assay. The levels of ET-1 and VEGF were dosed in cell culture supernatants at the beginning and during the culture. In ASC cultures, both ET-1 and VEGF were produced with and without osteogenic factors. ET-1 levels decreased, whereas high VEGF levels were constantly released over time (a, b). In SVF cultures, ET-1 showed an increasing trend of secretion with osteogenic factors, compared to a variable production in regular medium (c). VEGF levels increased until 30 days of culture, then they decreased with and without osteogenic factors (d).