Figure 1.

The effect of H₂O₂ on DBMSC proliferation determined by the MTS assay. After 24 and 48 h and as compared to untreated DBMSCs, the incubation of DBMSCs with various concentrations of H₂O₂ ranging from 1 to 200 μM did not induce a significant change in their proliferation, P > 0.05 (a and b) while their incubation with 50 and 100 μM of H₂O₂ for 72 h significantly increased their proliferation, ^∗P < 0.05 (c). The incubation of DBMSCs with 400 and 600 μM H₂O₂ for the indicated time significantly decreased their proliferation as compared to untreated DBMSCs, P < 0.05 (a and b). Experiments were carried out in triplicate using DBMSCs (passage 3) prepared from five individual placentae. Bars represent standard errors.