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Construction of a ΔpsbY mutant strain of Synechocystis 6803. A, Scheme for the insertional inactivation of the psbY gene. A 0.85-kb gentamycin-resistance cassette was inserted at a SspI site in the middle of the sml0007 ORF. Wild-type (WT) cells were transformed with this construct to generate the ΔpsbY strain. B, Southern-blot analysis of XmnI-digested chromosomal DNA from wild-type and ΔpsbY cells probed with a 32P-labeled PCR product corresponding to the sml0007 ORF.
