Table I.
Growth and photosynthetic properties of wild-type and ΔpsbY strains
Wild Type | ΔpsbY | |
---|---|---|
Doubling time (h) | ||
In BG11 | 20 ± 2 | 18 ± 3 |
In BG11 + Glc | 13 ± 1 | 11 ± 2 |
Electron transfer ratesa (μmol O2 mg−1 chlorophyll h−1) | ||
Whole chain (H2O–CO2) | 280 ± 40 | 260 ± 60 |
PSII (H2O–DCBQb/FeCNc) | 660 ± 160 | 560 ± 60 |
Relative PSII contentd | 100% | 82% |
Each value is the mean ± sd of at least three independent measurements.
Determined by polarographic measurements of rates of oxygen evolution.
DCBQ, 2,6-Dichloro-p-benzoquinone.
FeCN, K3Fe(CN)6.
Determined as the maximum flash-induced fluorescence yield normalized to the initial level of fluorescence, (Fmax − F0)/F0, in the presence of 20 mm hydroxylamine and 40 μm DCMU.