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. 2018 May 7;12:36. doi: 10.3389/fncir.2018.00036

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Copyright © 2018 Almada, Genewsky, Heinz, Kaplick, Coimbra and Wotjak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infusion of viral vectors into the SNr. (A) Viral vectors were injected into the SNr, resulting in the expression of either Channelrhodopsin-2 (ChR2)-mCherry (n = 10) or mCherry (n = 8) in the nigrotectal pathway. The optical fibers were placed above the SC. (B) Representative coronal photomicrographs showing the expression of ChR2-tdtomato in SNr somata, as well as in SNr terminals within the periaqueductal gray (PAG) matter and SC (blue: DAPI, red: tdtomato).