Figure 6.

Adoptive transfer of T follicular helper (Tfh) or B cells delayed remission of MOG_35–55-induced experimental autoimmune encephalomyelitis (EAE). (A) The process diagram of adoptive transfer experiments. Briefly, CD4⁺CXCR5⁺ Tfh-like cells and CD19⁺ B cells were, respectively, sorted from the spleens of MOG_35–55-immunized mice (donor) at 18 dpi. Isolated CD4⁺CXCR5⁺ T cells and/or CD19⁺ B cells were then transferred into pre-immunized mice (recipient) at 2 dpi. (B) The EAE score were measured in the following 55 days after antigenic challenge (n = 3 mice/group). (C) Cumulative score of each mouse in the 55 days. (D) Average score of each mouse in the 55 days. (E) The number of days for each mouse to score more than 4. All data were shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. (F) H&E and LFB staining for the spinal cords of the adoptive transferred mice.