Table 2.

Mutation types.

Gene-sgRNA	Clone	Alleles defined (N)	Mutation in each allele
			Small deletion	Small insertion	Small subs.	Large deletion	Large insertion	Invers.	Large subs.	Undef.
LFY-sg1	717	174	106 (60.9%)	58 (33.3%)	0 (0.0%)	2 (1.1%)	5 (2.9%)	0 (0.0%)	0 (0.0%)	3 (1.7%)
LFY-sg2		76	53 (69.7%)	20 (26.3%)	1 (1.3%)	1 (1.3%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	1 (1.3%)
LFY-sg1sg2		153	31 (20.3%)	15 (9.8%)	0 (0.0%)	98 (64.1%)	0 (0.0%)	8 (5.2%)	0 (0.0%)	1 (0.7%)
AG1-sg1		64	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)
AG2-sg1		8	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)
AG1-sg2		112	89 (79.5%)	19 (17.0%)	0 (0.0%)	2 (1.8%)	2 (1.8%)	0 (0.0%)	0 (0.0%)	0 (0.0%)
AG2-sg2		116	92 (79.3%)	23 (19.8%)	0 (0.0%)	0 (0.0%)	1 (0.9%)	0 (0.0%)	0 (0.0%)	0 (0.0%)
AG1-sg1sg2		177	121 (68.4%)	15 (8.5%)	4 (2.3%)	30 (16.9%)	1 (0.6%)	0 (0.0%)	0 (0.0%)	6 (3.4%)
AG2-sg1sg2		39	27 (69.2%)	4 (10.3%)	0 (0.0%)	4 (10.3%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	4 (10.3%)
LFY-sg1sg2	353	54	1 (1.9%)	2 (3.7%)	0 (0.0%)	49 (90.7%)	0 (0.0%)	1 (1.9%)	1 (1.9%)	0 (0.0%)
AG1-sg1sg2		53	37 (69.8%)	3 (5.7%)	0 (0.0%)	13 (24.5%)	0 (0.0%)	0 (0.0%)	0 (0.0%)	0 (0.0%)
AG2-sg1sg2		61	44 (72.1%)	9 (14.8%)	0 (0.0%)	7 (11.5%)	1 (1.6%)	0 (0.0%)	0 (0.0%)	0 (0.0%)
Total		1159

Rates of major classes of mutations from each gene-sgRNA combination. Undefined refers to insertion lines whose alleles were difficult to define by DSDecode. The most prevalent mutation type is highlighted in green and bold and the second most prevalent type in yellow and italics. Small refers to mutations of 15 bp or less. Invers., inversion; N, number; subs., substitution; Undef., undefined.