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Lipopolysaccharide (LPS)-stimulated inflammation was exacerbated in sirt1−/− macrophages. Wild-type (WT) and sirt1−/− macrophages were treated with LPS or PBS (NT), and then RT-PCR was used to assess the levels of IL-1β, IL-6, TNF-α, CCL2, and NOS2. **p < 0.01 compared with the LPS + WT group; n = 6.
