Figure 1.

F3 and NH36-vaccinated dendritic cells donor mice showed enhanced IDR and reduced clinical and parasitological impact of infection. IDR to the leishmanial antigen was measured at 24 h (A,B) and 48 h (C,D) after complete immunization with the F3 (F3sap) and the NH36 (NH36sap) vaccines, and after immunization and challenge. Parasite load in liver is expressed as LDU values (E) and total promastigotes/ml, in in vitro cultures of livers as determined by the limiting dilution assay method (F). Liver relative weight (G), and gain in corporal weight in grams (H) were recorded after euthanasia. Results represent the individual values of two-independent experiments (n = 6–7 C57BL/6 mice per treatment for each experiment). Horizontal full lines represent the mean values. Asterisks and horizontal lines show significant differences between treatments as disclosed by Mann–Whitney non-parametrical test.