Figure 9.

Cytokines expressed by CD4⁺ T lymphocytes in response to NH36. Effect of the F3 and NH36-vaccines on the frequencies of CD4⁺IL-2⁺ (A), TNF-α⁺ (D), IFN-γ⁺ (G), TNF-α⁺IL-2⁺ (B), TNF-α⁺IFN-γ⁺ (E), IL-2⁺IFN-γ⁺ (H), and IL-2⁺TNF-α⁺IFN-γ⁺-secreting T cells (C) in response to the NH36 antigen, on day 15 and 28 post challenge. The total CD4⁺ T cell frequencies are also represented (F). Bars represent the mean + SE values of two-independent experiments (n = 5 mice per treatment in each experiment). Asterisks and horizontal lines show significant differences between treatments as disclosed by Mann–Whitney non-parametrical test.