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. 2018 May 7;9:996. doi: 10.3389/fimmu.2018.00996

Figure 10.

Figure 10

The interferon regulatory factor 5 (IRF5) promoter risk insertion/deletion (indel) results in increased IRF5 transcription and specificity protein 1 (SP1) binding in myeloid cells. (A) Normalized activities of non-risk and risk indel promoter-driven luciferase transcription in various cell lines (left), fold change in luciferase activity due to the risk indel in various cell lines (center), and risk, non-risk, and mutated promoter-driven luciferase transcription in THP-1 cells (right). Results of three or four independent experiments are shown. (B) Electrophoretic mobility shift assay showing binding to the 3× and 4× CGGGG indel by various nuclear extracts (left), supershift assay showing SP1 binding to the 3× and 4× CGGGG indel in nuclear extracts of THP-1 cells (right). Results of three independent experiments are shown. (C) SP1 mRNA levels in various cell lines. Results of three independent experiments are shown. p < 0.05 by Kruskal–Wallis test [(A), center].