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. 2018 May 7;9:1000. doi: 10.3389/fimmu.2018.01000

Figure 8.

Figure 8

Soluble factors released by platelets mediate the modulation of surface molecules on monocytes. THP-1 cells were stimulated with supernatants collected from platelets incubated in presence or absence of Brucella abortus and assessed by flow cytometry. Monocyte population was identified in a FSC vs. SSC dot plot (A) and the CD54 surface expression was quantified within this region (B). Supernatants from bacteria cultured alone were used as control. Bars represent the arithmetic means ± SEM of three experiments. MFI, mean fluorescence intensity. THP-1 cells were incubated with supernatants of B. abortus-stimulated platelets in presence or absence of neutralizing Ab to soluble CD40L (sCD40L) (C) or platelet factor 4 (PF4) (E). THP-1 cells were stimulated with sCD40L (D), PF4 (F), or platelet-activating factor (PAF) (G) in their recombinant forms. (H) THP-1 cells were treated with supernatant collected from B. abortus-stimulated platelets in presence or absence of aspirin. (I) THP-1 cells were stimulated with a combination of sCD40L, PF4, and PAF. (J) THP-1 cells were treated with supernatant obtained from B. abortus-stimulated platelets in presence or absence of aspirin plus anti-sCD40L and anti-PF4 neutralizing antibodies. In all cases, CD54 surface expression was assessed by flow cytometry after the different treatments. Bars represent the times of CD54 induction ± SEM of three experiments. **P < 0.01; ***P < 0.001 vs. untreated. ###P < 0.001. NS, not significant. THP-1:PTL:Ba 1:100:100.