Statistically significant changes in pHc occur in columella cells of Arabidopsis following gravistimulation (t = 0 s). A, Tier 2 cells (n = 10) alkalinize immediately following gravistimulation. ●, Control; ▿, after gravistimulation. B, Tier 3 cells (n = 10) acidify 180 s following gravistimulation. ●, Control; ▿, after gravistimulation.