Fig. 6. Identification of BBP protein binders. (A). Structure of the tested BBP. (B). Structure of 27c determined by NMR. (C). Circular dichroism spectrum of 27c in aq. 10 mM phosphate buffer pH = 7.0 (black), in 25% trifluoroethanol in aq. 10 mM phosphate buffer pH = 7.0 (red), and in aq. 6 M guanidinium chloride (blue). (D). Percentage of BBP 27x/s/c detected by LC/MS of supernatant after incubation and centrifugation. Control = cell culture medium, 1 h, 4 °C; HeLa = HeLa cell lysate, 1 h, 37 °C; denat. HeLa = heat denaturated HeLa cell lysate, 1 h, 37 °C; HeLa + UV: HeLa cell lysate, irradiation with UV 310 nm for 10 min. Results were similar with 28x/s/c and 29x/s/c (data not shown) (E). Targets identified by CCMS. (F). MicroScale Thermophoresis of 27c with CALM1 (calmodulin). (G). Isothermal titration calorimetry of 27c with CALM1.