Figure 2.

Effect of exogenously supplied NAE 14:0 (0.1 mm) on the alkalinization of tobacco cell culture medium induced by different elicitors. A, Harpin 420 ng mL⁻¹ (▪), control (●), hairpin plus NAE14:0 (▴), and NAE14:0 (▾). B, Cryptogein 150 nm (▪), cryptogein plus NAE14:0 (▴), and control (●). C, Ergosterol 10 nm (▪), ergosterol plus NAE14:0 (▴), and control (●). The elicitors and NAEs were added to the culture medium as described in “Materials and Methods” and the pH was recorded every 2 min for 40 min. Controls and experimental treatments were carried out on the same population of cells. Results presented are representative; similar trends were observed in experiments repeated three times.