Table II.
The level of free amino acids in wild-type and AS8 antisense tobacco cells grown in complete or low-P medium for 5 d
| Amino Acid | Complete Medium
|
Low-P Medium
|
||
|---|---|---|---|---|
| Wild type | AS8 | Wild type | AS8 | |
| μmol g−1 dry wt | ||||
| Ala | 116 (16) | 7 (2) | 6 (1) | 25 (2) |
| Asn | 9 (2) | 8 (0.3) | 68 (3) | 68 (4) |
| Asp | 3 (1) | 10 (1) | 8 (0.3) | 12 (1) |
| Gln | 57 (8) | 67 (8) | 1,018 (18) | 546 (18) |
| Glu | 22 (6) | 38 (3) | 21 (1) | 31 (0.4) |
| Ser | 6 (1) | 13 (1) | 6 (2) | 67 (5) |
| Tyr | 3 (0.3) | 9 (1) | 14 (0.2) | 213 (8) |
| Totala | 265 (40) | 201 (5) | 1,232 (31) | 1,041 (20) |
Data are the average ± se (number in brackets) from three independent experiments on different subcultures of cells.
a
This is the sum of the free amino acid levels of all 20 of the protein amino acids (see also Fig. 6).