Fig. 5. QCM analysis of MIA-nanoparticle interaction. Change in frequency, Δf, and dissipation, D. Dotted lines represent dissipation and solid lines frequency. Red lines are MIA-1, gold lines Mia-2, blue lines control Adhiron, and grey lines blank experiments with no Adhiron. Phase A is injection of protein into the system, B and C are rinses with ultrapure water and PBS respectively. MNP are injected into the system during D, and Phase E is final rinsing with PBS. Time averaged frequency changes for MIA-1 at different overtone orders are also shown.