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. Author manuscript; available in PMC: 2018 May 14.
Published in final edited form as: Exp Eye Res. 2018 Jan 31;169:91–98. doi: 10.1016/j.exer.2018.01.024

Fig. 1. Axon density and thickness are reduced in response to acute DS.

Fig. 1

A. Representative images of a control and an acute DS cornea from d10 are presented. The ICNs have been visualized using an antibody against βIII tubulin. A minimum of 5 corneas for each variable were assessed. B. Images including those presented in 1A were used to quantify the axon density of the ICNs using Sholl analysis. The total number of corneas assessed (n) are indicated; both eyes of each animal were used. Each red or black circle represents the axon density of one cornea. Axon density decreased significantly relative to controls at d5 after MMC treatment and at d10 after both vehicle (veh) and MMC treatment. D. Representative images of corneas used to measure mean axon thickness are presented. E. Mean axon thickness was quantified and is presented. Axon thickness is reduced at d5 and d10 compared to controls. Bar in A = 500 µm; bar in D = 100 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)