Figure 3.

Northern and western analyses demonstrating that the seed is not directly involved in the perception of the light signal. A, From greenhouse-grown wild-type plants, total RNA was extracted from 15-DAP seeds obtained from an ear covered for 3 d with aluminum foil (AF) or from kernels obtained from a 25-DAP ear. Northern-blot analysis was carried out using the probes indicated on the right. rRNA stained with ethidium bromide is used as control of loading. Seeds were harvested at midday (white bar) or at midnight (black bar). B, Twenty micrograms of whole cell protein extracts per lane, made from the same seeds used for the RNA analysis of the aluminum foil experiment, were separated by 10% (w/v) SDS-PAGE and analyzed by western blot using a polyclonal O2 antibody. Seeds were harvested at midday (white bar) or at midnight (black bar). Apparent sizes are indicated at right in kD. Lane o2R is as indicated in Figure 1.