Figure 2.
Hinokitiol inhibits PDGF-BB and serum induced DNA synthesis in VSMCs. Serum-starved VSMCs were pretreated in the presence or absence of hinokitiol (1–10 μM), and then stimulated with (A) 10 ng/ml PDGF-BB or (B) 10% fetal bovine serum (FBS) for 48 h. DNA synthesis was assessed using BrdU incorporation assay kits. Data are presented as mean ± SEM (n = 3). *p < 0.05 compared to control group, #p < 0.05 compared to PDGF-BB or FBS stimulated cells. C – VSMCs (1 × 105 cells/cover slip) were placed on cover slips, allowed to adhere overnight and then serum starved for 24 h. The VSMCs were pre-treated in the presence or absence of hinokitiol (10 μM), and then stimulated with 10 ng/ml PDGF-BB for 48 h. BrdU positive VSMCs were directly observed by confocal microscopy. Representative immunofluorescence staining for BrdU was revealed in green and nuclear staining DAPI was revealed in blue. The white bar indicates 100 μm