Figure 3.

Metformin treatment precludes NGF-enhanced viability and reduces the number of A2780, HOSE and EA.hy926 cells.

Cells were stimulated with metformin (1 mM and 10 mM) and NGF (100 ng/ml) for 48 h. (a–f): viability of A2780, HOSE and EA.hy926 cells (n = 3, triplicate); (g–l) numbers of cells (n = 3, triplicate). Statistically significant differences are indicated as *p < 0.05; **p < 0.01; ***p < 0.001; ∆=p < 0.05. Statistical analysis, Kruskal–Wallis test and Mann–Whitney test respectively.

B, basal; HOSE, human ovarian surface epithelial; M1, metformin 1 mM; M10, metformin 10 mM; N100, NGF 100 ng/ml; NGF, nerve growth factor.