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Analysis of FITC-VLDL binding and uptake in CHO WT and mutant cell lines. CHO WT, SC, and Galnt11 KO (1C8 clone) were incubated with two different concentrations of FITC-VLDL (20 or 50 μg/ml) at 4 °C for binding (left panel) or 37 °C for internalization (right panel) for 4 h. The FACS analysis and quantification was performed as described in the legend to Fig. 4. The experiment was performed twice with similar results, and a representative data set is presented.
