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. 2018 Mar 19;293(19):7195–7208. doi: 10.1074/jbc.RA118.002639

Figure 3.

Figure 3.

Gene expression of AP surface and soluble regulatory protein genes in unstimulated and TNF-stimulated BMVECs relative to GMVECs. The mRNA levels of the genes for three membrane-bound regulatory receptors (CD46, THBD, and CD55) and two soluble regulatory proteins (CFH and CFI) were evaluated by RT-qPCR in unstimulated BMVECs and GMVECs (A) and in TNF-stimulated BMVECs and GMVECs (B). RNA was extracted from unstimulated BMVECs and GMVECs that were maintained in serum-free medium for 24 h and from TNF-stimulated cells after incubation with 10 ng/ml TNF for 48 h (24 h in complete medium and 24 h in serum-free medium). Fold-changes in BMVEC mRNA levels were calculated relative to the mRNA levels in GMVECs. RNA was extracted in 4–6 separate experiments from each cell type. Data are means plus S.D. from RT-qPCR runs with triplicate measurements. GAPDH was used for normalization. *, p < 0.05; **, p < 0.001.