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Levels of the soluble AP regulatory protein, FH, in the supernatant of unstimulated and TNF-stimulated GMVECs and BMVECs. Supernatant from unstimulated GMVECs (n = 4) and BMVECs (n = 4) in T-75 flasks was collected after 24 h in serum-free media and assayed for FH by ELISA. The same flasks were then stimulated for 48 h with 10 ng/ml TNF (24 h in complete media and 24 h in serum-free media), and the 24-h supernatant was again collected and assayed for FH. *, p < 0.05.
