Figure 2.

5/6 nephrectomized (CKD) mice on high-fat, high-cholesterol diet (HFD) demonstrate increased atherosclerosis compared with control mice on HFD and CKD mice on low-fat diet. Representative Oil Red O staining and morphometry of aorta and main branches from male LDLr^−/− mice are shown. A and F, control (CTL) mice on low-fat diet (LFD) for 12 and 24 weeks. B and G, 5/6 nephrectomized CKD mice on LFD for 12 and 24 weeks. C and H, CTL mice on HFD for 12 and 24 weeks. D and I, CKD mice on HFD for 12 and 24 weeks. E and J, analysis of total atherosclerotic lesion area ratio between the four groups at 12 and 24 weeks reveals increases in lesional ratio (ratio of Oil Red O-stained area to the total surface area of en face section of the aortic tree expressed as a percentage) in the CKD and HFD groups. The CKD HFD group has the most extensive lesional area among the four groups that is significantly higher when compared with CTL HFD group. (*, p < 0.05; number of mice is shown in parentheses.)