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. 2018 Mar 26;293(19):7238–7249. doi: 10.1074/jbc.RA117.000559

Figure 5.

Figure 5.

Increased myeloperoxidase and its products present in atherosclerotic lesions of 5/6 nephrectomized mice on high-fat, high-cholesterol diet (HFD). Representative immunohistologic staining for myeloperoxidase (MPO) in the aortic cross-sections from male LDLr−/− mice after 24 weeks of HFD is shown. Magnification ×1000. When compared with control (CTL-HFD) mice (A), intense staining for MPO was observed in the intimal lesions of 5/6 nephrectomized (CKD-HFD) mice (B) atherosclerotic lesions. Mass spectrometric quantification of oxidized amino acids in aortic proteins in male LDLr−/− mice on 24 weeks of HFD or LFD. C, 3-chlorotyrosine; D, 3-nitrotyrosine; and E, o,o′-dityrosine expressed as ratios to precursor amino acid tyrosine in μmol/mol (n = 4–7 each); F–H show the correlation using Pearson's correlation between the three oxidation products (*, p < 0.05).