Figure 4.

Northern-blot analyses of CAH1/ARS chimeric transcripts in the transformants CAO2-1 and CAO3-1. A and B, Cells grown under high-CO₂ conditions were transferred to low-CO₂ conditions and total RNA was isolated 0, 1, 2, 4, and 6 h after the change in CO₂ level. Ten micrograms of RNA from each of the transformants was electrophoresed in a denaturing agarose gel, blotted onto a membrane, and hybridized with ³²P-labeled p-CA1-5′ (A) or ARS cDNA (B). CAH1/ARS represents the 2.5-kb transcripts from the chimeric constructs. C, CAO2-1 and CAO3-1 were grown under high-CO₂ conditions in the light and subsequently transferred to low-CO₂ conditions in the light (L) or dark (D). Total RNA was isolated 4 h after transfer to each of these conditions. The oligonucleotide probe p-CA1-5′ was used for hybridization.