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. 2018 Mar 28;293(19):7329–7343. doi: 10.1074/jbc.RA117.001049

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© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — GRP94 masks ER-resident PCSK9 from GRP78. A, coimmunoprecipitation experiments were carried out on cells transfected with VP and its ER-retention variant (VP^G17V) and compared with that of cells transfected with PCSK9 and its ER-retention variant (PCSK9^Q152H). VP was immunoprecipitated using an anti-GFP capture antibody, whereas PCSK9 was captured using an anti-V5 antibody; effective capture was confirmed by immunoblotting (IB) IPs with anti-GFP and anti-PCSK9 antibodies, respectively. B and C, interactions characterized in A were confirmed by IP of endogenous GRP94 and GRP78 in cells transfected with V5-PCSK9 and GFP-VP, respectively. D, binding of GRP78 to PCSK9^WT and PCSK9^Q152H was also examined in the presence of siGRP94. B, C, and D, whole cell lysates were used as positive controls for antibody staining.