Figure 2.

TIFA potentiates DNA damage–induced NF-κB activation and secretion. a, NF-κB luciferase reporter was transfected to HeLa cells stably expressing FLAG-TIFA or control cells. Renilla vector was also transfected simultaneously and served as transfection control. After treatment of cells with ETO at the indicated times, the cells were harvested for luciferase activity assay. Data were represented as the mean ± S.D. from three independent experiments. **, p < 0.01 (Student's t test). b, the HeLa cells were transfected with control or FLAG-TIFA expression vectors. After 2 days, the cells were further treated with vehicle or ETO for 6 h. The cell lysate was then harvested for Western blot analysis with the indicated antibodies. p-IκBα indicates the antibody against IκBα phosphorylation on serine 32 and serine 36. c, the total mRNA was prepared from cells described in b and the mRNA levels of indicated genes were examined using quantitative RT-PCR analysis. Data were represented as the mean ± S.D. from eight independent experiments. **, p < 0.01 (Student's t test). d, the HeLa cells were transfected with control or FLAG-TIFA expression vectors and further treated with or without ETO. The secretory levels of interleukin (IL)-6 and IL-8 were then measured by ELISA. Data were represented as the mean ± S.D. from three independent experiments. **, p < 0.01 (Student's t test).