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. 2018 Mar 9;293(19):7476–7485. doi: 10.1074/jbc.RA118.002233

Figure 1.

Figure 1.

Human TRIM24 is SUMOylated. A, WCL from MCF7 cells prepared in lysis buffer with 1% SDS or NEM and subjected to TRIM24 Western blot analysis using p84 as loading control. B, TRIM24 is modified by SUMO1 and SUMO2/3. WCL from HEK293T cells were subjected to immunoprecipitation, using control IgG and TRIM24 antibody, followed by Western blot analysis with TRIM24, SUMO1, and SUMO2/3 antibodies. C, WCL from HEK293T cells transfected with FLAG-TRIM24 and HA-SUMO1 were used for FLAG immunoprecipitation (IP) followed by Western blot analysis with FLAG and HA antibodies. *, nonspecific band. D, TRIM24 is deSUMOylated by SENP1. HEK293T cells were transfected with FLAG-SENP1 and/or HA-SUMO1 plasmids followed by Western blot analysis using TRIM24 and FLAG antibodies. E, time course showing in vitro SUMOylation of bacterially expressed and purified GST-TRIM24 protein.