Table 3.
Kd values for sodium and chloride
Kd values were derived by nonlinear regression analysis of Na+ and Cl− dose–response curves, obtained by measuring [3H]dopamine uptake into transiently transfected COS-7 cells during equimolar titration of 200 mm NaCl against choline chloride and sodium gluconate (final Na+ and Cl− concentrations of 200, 130, 80, 40, 20, 10, 5, and 0 mm). *, p < 0.05; ***, p < 0.001. One-way ANOVA with Dunnett's multiple comparisons test (n = 3 for variants and n = 6 for DAT WT, with triplicate determinations). Kd values for D421N could not be reliably fitted, because of the linear nature of the ion dependency curves.
| Na+ Kd (mm), mean ± S.E. | Cl− Kd (mm), mean ± S.E. | |
|---|---|---|
| WT | 29.1 ± 1.2 | 31.5 ± 1.6 |
| I312F | 136 ± 44* | 221 ± 38*** |
| T356M | 145 ± 64* | 71.6 ± 16 |
| D421N | >WT | >WT |
| A559V | 25.7 ± 0.56 | 30.2 ± 5.8 |
| E602G | 31.5 ± 0.97 | 27.8 ± 2.3 |
| R615C | 31.2 ± 0.41 | 32.6 ± 3.7 |