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. 2018 Mar 28;293(19):7300–7314. doi: 10.1074/jbc.RA117.000775

Figure 2.

Figure 2.

Modified mRNA encoding the short isoform of FUT4 (FUT4SHORT) enforces CD15 expression in transfected hMSCs, whereas the long isoform of FUT4 (FUT4LONG) has no effect. A, schematic diagram of long and short FUT4 isoforms including 5′ and 3′ UTR, start codons (ATG), and stop codons. B, schematic diagram of modRNA constructs including the coding sequence from the long and short FUT4 isoforms. C, Lewis X (CD15) levels were measured by flow cytometry on hMSCs transfected 2 days prior with FUT4LONG modRNA or FUT4SHORT modRNA. Statistics performed were: one-way ANOVA (p < 0.0001) plus Dunnett's post-test to compare all stained samples with unstained control (**, p < 0.01). Individual data points are depicted, with error bars representing S.D.