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. 2018 Mar 28;15(6):8761–8769. doi: 10.3892/ol.2018.8363

Figure 3.

Figure 3.

GEM enhances shPPP5C-induced PANC-1 cell cycle arrest and apoptosis. (A) Cell cycle distribution was analyzed by flow cytometry. Representative images of shCon, shPPP5C and the combined treatment of GEM and shCon or shPPP5C measured by fluorescence-activated cells sorting analyses. (B) Quantification and statistical analysis of the cell percentages in the different cell cycle phases in shCon group. (C) Quantification and statistical analysis of the cell percentages in the different cell cycle phases in shPPP5C group. (D) Proportion of cells in sub-G1 phases. (E) Statistical analysis of the proportion of cells in early and late apoptosis. (F) Representative images of the flow cytometry of PANC-1 cells treated with shCon, shPPP5C or combined treatment of GEM and shCon or shPPP5C. Data are presented as the mean ± standard deviation of 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001. GEM, gemcitabine; shPPP5C, short hairpin serine/threonine protein phosphatase 5; shCon, short hairpin control.