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. 2018 Mar 28;15(6):8761–8769. doi: 10.3892/ol.2018.8363

Figure 4.

Figure 4.

Effects of PPP5C-knockdown on apoptosis pathway-associated molecular expression. (A) The levels of caspase-3, PARP, JNK, p-JNK, p38 and p-p38 protein in PANC-1 cells were detected following PPP5C silencing by western blot analysis. (B) The levels of p-p53 (ser315) and p53 protein in PANC-1 cells were analyzed following PPP5C silencing by western blot analysis. (C) The expression of JNK, p-JNK, p38 and p-p38 proteins in PANC-1 cells transduced with shCon, shPPP5C or the combined treatment of GEM and shPPP5C or shCon was analyzed by western blot analysis. (D) Proposed model of the role served by PPP5C silencing in cell apoptosis. GAPDH was used as an internal loading control. PPP5C, serine/threonine protein phosphatase 5; JNK, c-Jun N-terminal kinases; shCon, short hairpin control; shPPP5C, short hairpin PPP5C; GEM, gemcitabine; p-, phosphorylated-; t-, total-.