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. 2018 Mar 29;15(6):8681–8686. doi: 10.3892/ol.2018.8372

Figure 3.

Figure 3.

POSTN promotes the migration of TAMs. (A) Transwell assay showing comparison of TAMs migration toward CM from NSCCs and ICSCs in HCCC-9810 cells (magnification, ×400) and (B) the graphical analysis. Data are presented as mean ± SD (n=3). *P<0.05, migrated TAMs towards ICSC CM vs. NSCC CM. The analysis identified that the increased TAMs cell migration toward ISCCs CM relative to NCSCs CM. (C) Comparison of TAMs migration toward ICSC CM or following treatment with anti-POSTN (10 µg/ml) antibody or IgG (magnification, ×400) and (D) its graphical analysis. Data are presented as mean ± SD (n=3). *P<0.05, migrated TAMs towards to α-POSTN ICSC CM vs. ICSC CM and IgG ICSC CM. (E) Comparison of invading TAMs toward NSCC CM or following treatment with rPOSTN (0.2 µg/ml) or IgG (magnification, ×400) and the (F) graphical analysis. Data are presented as mean ± SD (n=3). *P<0.05, migrated TAMs towards to rPOSTN NSCC CM vs. NSCC CM. POSTN, periostin; TAM, tumor-associated macrophage; NSCCs, non-stem cancer cells; ICSCs, intrahepatic cholangiocarcinoma stem cells; IgG, immunoglobulin G; r, recombinant; CM, conditional medium; SD, standard deviation; Ig, immunoglobulin.

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