Figure 5.

Immunohistochemical assessment of α-SMA-positive microvessel density in xenograft tumors in response to bevacizumab treatment. (A) Representative microphotographs of α-SMA-immunostained tumor sections obtained from untreated control mice and from mice treated with bevacizumab or saline. Scale bar=50 µm. (B) Quantification of intratumoral microvessel density by α-SMA immunostaining. Each bar represents the mean α-SMA-positive intratumoral microvessel density count ± SD. A significant decrease in α-SMA-positive microvessel density was observed with bevacizumab treatment compared with the untreated control or saline-treated tumors (P<0.01). **P<0.01. α-SMA, α-smooth muscle actin.