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. 2018 Apr 4;15(6):8516–8526. doi: 10.3892/ol.2018.8431

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Copyright: © Slater et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Putative cancer stem cell identification by Hoechst 33342 dye exclusion in SW480 and SW620 cells. The proportion of cells representing the SP in the SW480 and SW620 cell lines was determined using Hoechst 33342 dye efflux. (A) Representative dot plots indicating the SP within SW480 and SW620 cells stained with 5 µg/ml Hoechst 33342. The SP was detected by the loss in the fluorescent population (H^blue-/H^red−) upon treatment with the ABCG2 inhibitor, verapamil (50 µM). (B) Putative cancer stem cell populations representative of the average percentage SP observed (mean ± standard deviation) from triplicate independent experiments measured as: SP = (% cells H^blue−/H^red− without verapamil) - (% cells H^blue−/H^red− with verapamil). No significant differences were identified when comparing the SP of the SW480 and SW620 cell lines (t-test with Welch's correction). SP, side population.