Figure 3.

Confocal laser-scanning images of cell fusion between hUCMSCs and tumor cells. Tumor cells and hUCMSCs were stained with DiO (green) and DiD (red), respectively. All samples were counterstained with Hoechst 33342 (blue) to indicate the nucleus. After co-culture for 72 h, the hUCMSCs were revealed to have merged into A549 or BEL7402 tumor cells, as illustrated by the existence of hybrids with double nuclei and yellow cell membranes (DiO and DiD double-stained cells indicated by the white arrow) in the representative images. Scale bar, 20 µm; magnification, ×400.