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The relevance of allelic frequency and coefficient of variation. Bars indicate values of mean ± standard deviation of allelic frequencies of reference materials determined by the ddPCR assay in quadruplicate for EGFR (A) 19del, (B) L858R, and (C) T790M mutations. The dots represent the coefficient of variation (%) of the allelic frequencies of reference materials determined by the NGS-based cancer IVDs from the eight participants.
