Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 14;5(1):e000259. doi: 10.1136/lupus-2018-000259

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

PMC Copyright notice

TLR9 deficiency promotes aberrant B cell activation and myeloid expansion in imiquimod-induced autoimmunity. WT and Tlr9^-/- mice were treated with imiquimod for 3 weeks. (A) Splenocytes were isolated and stained for CD45, CD19, CD21 and CD23 for MZ B cells. (B) Splenocytes were isolated and stained for CD45, CD11c, B220 and PDCA-1. Total pDC numbers were calculated. (C) mRNA levels of MX-1 in the spleen. (D) mRNA levels of ISG15 and IFIT1 in the spleen. (E) Splenocytes were isolated and stained for CD45, CD11b, Ly-6G for neutrophils. (F) Splenocytes were isolated and stained for CD45, CD11b, CD11c for CD11b⁺CD11c⁺ myeloid cells. (G) Splenocytes were isolated and stained for CD45, CD11b, Ly-6C and Ly-6G for proinflammatory monocytes. (H) mRNA levels of IL-1β, TNF-α and IL-12 p35 in the spleen. (I) Splenocytes were isolated and stained for CD45, CD11b and intracellularly stained for TNF-α and IL-12 p35 (n=6/group). cDC, conventional dendritic cell; IL, interleukin; MZ, marginal zone; pDC, plasmacytoid dendritic cell; TNF-α, tumour necrosis factor alpha; TLR, toll-like receptor; WT, wild type.