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M2-like macrophages promote the apoptotic activity in M1-like macrophages. Macrophages were isolated from control mice (M0 macrophages), then polarized into M1 or M2 phenotype using mouse IFN-γ or IL-4, respectively. Conditioned media (CM) from M0, M1, M2 macrophages were harvested and applied to M1 macrophages. Cell apoptosis was evaluated by immunostaining for cleaved caspase-3.
