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M2-like macrophages protect HepG2 cells from apoptosis. Human monocyte-derived macrophages were isolated from PBMC, then polarized into M1 or M2 phenotype using human IFN-γ or IL-4, respectively. M0, M1, M2 CM were incubated with HepG2 cells for 6 hours, then apoptosis was induced by TNF-α/D-GalN for 12 hours. Cell apoptosis was assessed by flow cytometry. * P<0.05, ** P<0.01, *** P<0.001.
