Fig. 3. Absorbance and emission spectra of the most responsive probes carrying (A) BO, (B) TO, (C) QV, (D) QB and (E) TR. Spectra are corrected for probe concentration. Conditions: 0.5 μM probe and 5 eq. RNA target, when added, in PBS at 37 °C. BO: λex = 455 nm; TO: λex = 485 nm; QV: λex = 530 nm; QB: λex = 560 nm; λex = 610 nm; slitex = 5 nm, slitem = 5 nm. Analytical data, absorbance and emission spectra of all probes are provided in the ESI (Table S1–S6 and Fig. S1–S5†).
