Fig. 4. Absorption (dashed) and emission spectra (solid) of QB-FIT probe vasa-QB-1 in complex with complementary vasa-1-RNA with (blue) and without (black) LNA. Conditions: 0.5 μM probe and 2.5 μM RNA target in PBS at 37 °C; λ_ex = 560 nm; slit_ex = 5 nm, slit_em = 5 nm.