Fig. 5. Simultaneous detection of three RNA targets. The arrows indicate time points when probes or targets were added to the buffer. LNA nucleotides are indicated by subscript “L”, 2′-O-Me nucleotides are underlined. Conditions: 0.5 μM of each probe and 3 eq. of complementary target in PBS-buffer, BO (blue line): λex = 460 nm, λem = 485 nm, TO (green line): λex = 505 nm, λem = 530 nm, QB (red line): λex = 575 nm, λem = 600 nm, slitex = 5 nm, slitem = 5 nm, 37 °C.
