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Cardiac specific markers miRNA level and the protein level of GATA4 and β-MHC. (A–D) Nkx2.5, GATA4, MEF2c, and β-MHC miRNA level at days 3, 6, 9, and 12 after cell transfection or 5-azacytidine treatment, respectively. (E) Western blotting showed the expression of GATA4 and β-MHC at day 12 after cell transfection or 5-azacytidine treatment. (F) GATA4 and β-MHC relative expression; * p<0.05, n=3.
