Table 1.
Primers | Direction | Sequences (5′-3′) |
---|---|---|
Nkx2.5 | Forward | AAGCAACAGCGGTACCTGTC |
Reverse | ACTTGTAGCGACGGTTCTGG | |
GATA4 | Forward | ATGCCTGTGGCCTCTATCAC |
Reverse | TGGTGGTAGTCTGGCAGTTG | |
MEF2c | Forward | TCCATCAGCCATTTCAACAA |
Reverse | AGTTACAGAGCCGAGGTGGA | |
β-MHC | Forward | CAGCAGTTCTTCAACCACCA |
Reverse | TCTCGATGAGGTCAATGCAG | |
GAPDH | Forward | GCCATCACTGCCACTCAGAA |
Reverse | GGCATGTCAGATCCACAACG | |
miR-199b-5p | (RT primer) | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTG |
Forward | GGCCCAGTGTTTAGACTACC | |
Reverse | CAGTGCGTGTCGTGGAGT | |
U6 | (RT primer) | AACGCTTCACGAATTTGCGT |
Forward | GTGCTCGCTTCGGCAGCACATATAC | |
Reverse | AAAAATATGGAACGCTTCACGAATTTG |