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Site-directed glycosylation of peptide/protein with uniform eukaryotic N-glycan. a. Chemically synthesized GlcNAc-O-peptide can be glycosylated by Endo M N175Q with complex-type glycan oxazoline (SCT-oxa) as a donor. b. GlcNAc-O-protein can be generated by coexpression with OGT and then linked with homogeneous eukaryotic N-glycan.
