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. Author manuscript; available in PMC: 2018 May 14.
Published in final edited form as: Bioconjug Chem. 2016 Aug 18;27(9):1972–1975. doi: 10.1021/acs.bioconjchem.6b00385

Figure 3.

Figure 3

Glycosylation of bovine α-Crystallin-A (Crys-A) and identification by immunoblot and in-gel digestion plus mass spectrometry. a. Immunoblot analysis of GlcNAc-O-Crys-A and glycan-O-Crys-A. (i) Anti-O-GlcNAc antibody is a primary antibody to detect GlcNAc. (ii). Anti-His antibody is a primary antibody to detect His-tag in Crys-A. b. In-gel digestion by trypsin for mass spectrometric characterization of glycan-Crys-A, calculated 4754.9837, found [M+5H]5+ = 952.4069, [M+6H]6+ = 793.8413. MS/MS characterization can be found in Figure S4.